学习经历
2004.09-2009.07 中国科学院物理研究所 博士 软物质物理与生物物理
2000.09-2004.07 山东大学物理学院 学士 物理学基地班
工作经历与任职情况
2020.9- ：教授，博士生导师 武汉大学生命科学学院
2015.11-2020.8：研究员，博士生导师 武汉大学生命科学学院，武汉大学高等研究院
2010.03-2015.10：研究科学家 新加坡-MIT联合科研中心生物系统与微观力学实验室
2009.07-2010.03：专利审查员 国家知识产权局
荣誉与奖励
湖北省高层次人才青年项目入选者（2018）
国家高层次人才青年项目入选者（2022）
主要研究领域及兴趣
使用单分子磁镊技术和单分子FRET技术，揭示RNA、DNA、蛋白质等生物大分子的动态结构及其相互作用。通过结构生物信息学方法，探索生物大分子的结构动态和功能机制。我们还发展了DNA力学探针，用于临床场景的分子检测和单分子细胞力学检测。当前的研究重点包括DNA甲基化、单分子检测技术、核小体和染色质的动态调控。
承担课程（本科生、研究生）
主讲课程：《生物物理学》、《高等生物物理学》、《单分子生物学》、《生物化学实验》
主持课题项目：
张兴华 教授
国家自然科学基金（2017、2018、2020、2024）
国家高层次人才计划青年项目基金（2022）
杨亚军 副研究员
国家自然科学基金（2022）、国家博士后特别资助（2024）、弘毅博士后（2023）
赵晓聪 助理研究员
国家博士后特别资助（2023）、弘毅博士后（2023）
田馥嘉
尖端人才引进计划（2023）、弘毅博士后 （2023）
代表性成果(#为共同第一作者，*为通讯作者)
通讯作者论文：
1. Yuru Hu#, Hongyun Li#*, Chen Zhang#, Jingjing Feng#, Wenxu Wang, Wei Chen, Miao Yu, Xinghua Zhang*, Zheng Liu*, "DNA-based ForceChrono Probes for Deciphering Single-Molecule Force Dynamics in Living Cells", Cell, 187(13), 1–15 (2024)
2. Chen Zhang#, Fu-Jia Tian#, Hong-Wei Zuo#, Wei Wei, Qi-Yuan Qiu, Zhi-Jie Tan, Yan Zhang*, Wen-Qiang Wu, Liang Dai* and Xing-Hua Zhang*, "Counterintuitive DNA destabilization by monovalent salt at high concentrations", Nature Communications in press (2024)
3. Yun-Long Chen* and Xing-Hua Zhang*, "Effects of imidazolium-based ionic liquids on the elasticity of DNA revealed by magnetic tweezers", Journal of Molecular Liquids, 410: 125646 (2024)
4. Er-Chi Zhou#, Hang Fu#, Hao-Ze Wang, Ya-Jun Yang, Xing-Hua Zhang*, "Converting multiple to single DNA-tethered beads and removing one-end-tethered only DNA in high-throughput stretching", ACS Sensors, https://doi.org/10.1021/acssensors.4c02585 (2024)
5. Bohua Yin, Shaoxi Fang, Bin Wu, Wenhao Ma, Daming Zhou, Yajie Yin, Rong Tian, Shixuan He, Wanyi Xie*, Xinghua Zhang*, Zuobin Wang*, Deqiang Wang*, "Directly Characterizing the Capture Radius of Tethered Double-Stranded DNA by Single-Molecule Nanopipette Manipulation", ACS Nano 18(40): 27962−27973 (2024)
6. Chen-Chen Zheng#, Yun-Long Chen#, Hai-Long Dong, Xing-Hua Zhang*, Zhi-Jie Tan*, "Effect of ethanol on the elasticities of double-stranded RNA and DNA revealed by magnetic tweezers and simulations", The Journal of Chemical Physics 161: 075101 (2024)
7. Peng Chen#, Jin Zhou#, Huan Liu#, Erchi Zhou#, Boxiao He, Yankang Wu, Hongjian Wang, Zaiqiao Sun, Chonil Paek, Jun Lei, Yongshun Chen*, Xinghua Zhang*, Lei Yin*, “Engineering of Cas12a nuclease variants with enhanced genome-editing specificity”, PLoS Biology, 22(3): e3002514 (2024)
8. Hai-Long Dong#, Chen Zhang#, Liang Dai, Yan Zhang*, Xing-Hua Zhang*, and Zhi-Jie Tan*, “The origin of different bending stiffness between double-stranded RNA and DNA revealed by magnetic tweezers and simulations.”, Nucleic Acids Research, 52(5):2519-2529 (2024)
9. Ya-Jun Yang, Hang Fu, Xiao-Lu Li, Hong-Yu Yang, Er-Chi Zhou, Cheng-Yu Xie, Shu-Wen Wu, Fan He*, Yan Zhang* and Xing-Hua Zhang*, “A mutation-sensitive, multiplexed and amplification-free detection of nucleic acids by stretching single-molecule tandem hairpin probes.”, Nucleic Acids Research, 51(17): e90 (2023)
10. Fu-Jia Tian#, Chen Zhang #, Erchi Zhou#, Hai-Long Dong, Zhi-Jie Tan, Xing-Hua Zhang* and Liang Dai*， “Universality in RNA and DNA deformations induced by salt, temperature change, stretching force, and protein binding.” PNAS, 120 (20) e2218425120 (2023)
11. Xiao-Cong Zhao#, Hai-Long Dong#, Xiao-Lu Li, Hong-Yu Yang, Xue-Feng Chen, Liang Dai, Wen-Qiang Wu, Zhi-Jie Tan* and Xing-Hua Zhang*, “5-methyl-cytosine stabilizes DNA but hinders DNA hybridization revealed by magnetic tweezers and simulations.” Nucleic Acids Research, 50 (21): 12344–12354 (2022)
12. Chen Zhang#, Fujia Tian#, Ying Lu, Bing Yuan, Zhi-Jie Tan, Xing-Hua Zhang* and Liang Dai*, “Twist-diameter coupling drives DNA twist changes with salt and temperature.” Science Advances, 8(12), abn1384 (2022)
13. Xiao-Wei Qiang#, Chen Zhang#, Hai-Long Dong#, Fu-Jia Tian, Hang Fu, Ya-Jun Yang, Liang Dai, Xing-Hua Zhang*, Zhi-Jie Tan*, “Multivalent cations reverse the twist-stretch coupling of RNA.” Physical Review Letters, 126, 108103. (2022)
14. Xuejie Wang#, Yang Dong#, Xiaocong Zhao#, Jinbao Li, Jordan Lee, Zhenxin Yan, Shuangshuang Yang, Wenqiang Wu, Ximiao Hou, Guangxue Liu, Yueyue Zhang, Lun Song, Gang Cai, Qing Li, Grzegorz Ira, Xinghua Zhang*, Xuefeng Chen*, “Rtt105 promotes high-fidelity DNA replication and repair by regulating the single-stranded DNA binding factor RPA.” PNAS, 118 (25): e2106393118 (2021)
15. Ya-Jun Yang, Hai-Long Dong, Xiao-Wei Qiang, Hang Fu, Er-Chi Zhou, Chen Zhang, Lei Yin, Xue-Feng Chen, Fu-Chao Jia, Liang Dai, Zhi-Jie Tan, and Xing-Hua Zhang*. “Cytosine methylation enhances DNA condensation revealed by equilibrium measurements using magnetic tweezers”. Journal of the American Chemical Society. 142 (20): 9203–9209 (2020)
16. Hang Fu#, Chen Zhang#, Xiao-Wei Qiang#, Ya-Jun Yang, Zhi-Jie Tan* and Xing-Hua Zhang*. “Opposite effects of high-valent cations on the elasticities of DNA and RNA duplexes revealed by magnetic tweezers.” Physical Review Letters. 124(5): 058101 (2020)
17. Chen Zhang#, Hang Fu#, Yajun Yang#, Erchi Zhou, Zhijie Tan, Huijuan You, Xinghua Zhang*. “The Mechanical Properties of RNA-DNA Hybrid Duplex Stretched by Magnetic Tweezers”. Biophysical Journal. 116 (2): 196-204 (2019) (Selected as the best paper of 2019)
18. Ya-Jun Yang#, Lun Song, Xiao-Cong Zhao, Chen Zhang, Wen-Qiang Wu, Hui-Juan You, Hang Fu, Er-Chi Zhou, Xing-Hua Zhang*. “A Universal Assay for Making DNA, RNA, and RNA–DNA Hybrid Configurations for Single-Molecule Manipulation in Two or Three Steps without Ligation”. ACS synthetic biology. 8(7): 1663-1672 (2019)
19. Ju-Hui Liu #, Kun Xi #, Xi Zhang, Lei Bao, Xing-Hua Zhang*, Zhi-Jie Tan*. “Structural Flexibility of DNA-RNA Hybrid Duplex: Stretching and Twist-Stretch Coupling”. Biophysical Journal. 117 (1): 74-86 (2019)
20. Xiao-Cong Zhao #, Hang Fu, Lun Song, Ya-Jun Yang, Er-Chi Zhou, Guang-Xue Liu, Xue-Feng Chen, Zhuo Li, Wen-Qiang Wu, Xing-Hua Zhang*, “S‑DNA and RecA/RAD51-Mediated Strand Exchange in Vitro”. Biochemistry. 58 (15): 2009-2016 (2019)
21. Xiao-Cong Zhao#, Bin Wu#, Ya-Jun Yang#, Qi-Yuan Qiu#, Liu Wang, Ya-Peng Xu, Ying Li, Han Gong, Jing-Yao Shi, Hang Fu, Lun Song, Xue-Feng Chen, Shi-Shen Du, Shao-Ran Zhang, Qi Zong, Xu-Guang Xi, Liang Dai, Yan Zhang*, Wen-Qiang Wu*, and Xing-Hua Zhang*, "Dam methylation of DNA promotes both RecA assembly and homologous pairing", Nature Chemical Biology peer review (2024)
22. Fujia Tian#, Chen Zhang#, Hongwei Zuo, Xing-Hua Zhang* and Liang Dai*, "Counterintuitive DNA deformation induced by multivalent ions through entropic pulling effect", Physical Review Letters peer review (2024)
第一作者论文：
1. Xinghua Zhang, Yuanyuan Qu, Hu Chen, Ioulia Rouzina, Shengli Zhang, Patrick S. Doyle*, and Jie Yan*, “Interconversion between three overstretched DNA structures.” Journal of the American Chemical Society, 136(45): 16073-16080 (2014)
2. Xinghua Zhang, Hu Chen, Shimin Le, Ioulia Rouzina, Patrick S. Doyle*, and Jie Yan*, “Revealing the competition between peeled ssDNA, melting bubbles and S-DNA during DNA overstretching by single-molecule calorimetry”, PNAS, 110(10): 3865-3870 (2013)
3.Xinghua Zhang, Hu Chen, Hongixa Fu, Patrick S. Doyle*, and Jie Yan*, "Two distinct overstretched DNA structures revealed by single-molecule thermodynamics measurements", PNAS, 109 (21): 8103-8108 (2012)
发明专利：
202310992413.1 《一种分子检测方法》
招生招聘
每年接收2-3名硕士生，2名博士生，1-2名博士后。
