Research Associate
Laboratory of RNA Splicing and Diseases
Department of Biochemistry, College of Life Sciences
Wuhan University
Wuhan 430072, China
Tel: 027-68789348
E-mail: fanyujie@whu.edu.cn
Research Interests:
As an essential step of RNA processing and maturation, RNA splicing is critical for the regulation of gene expression in higher eukaryotes. On one hand, the dynamic assembly of the spliceosome and precise splicing regulation ensure normal physiological processes such as cell differentiation and organ development. On the other hand, mutations of spliceosomal components, RNA cis-elements, and trans-factors lead to various human diseases including cancers and neurodegeneration. We are interested in the mechanism of splicing and its regulatory roles in development and diseases and currently focus on:
1) Splicing regulation in the development of nervous and reproductive systems;
2) The mechanism of circular RNA biogenesis and their regulatory roles in development and diseases.
Representative Publications （# co-first author, * corresponding author）
3) Li H#, Ding Z#, Fang ZY#, Long N, Ang HY, Zhang Y, Fan YJ*, and Xu YZ*. Conserved intronic secondary structures with concealed branch sites regulate alternative splicing of poison exons. Nucleic Acids Research, 2024, DOI: 10.1093/nar/gkae185, PMID: 38499485.
4) Wang M#, Liang AM#, Zhou ZZ, Pang TL, Fan YJ*, and Xu YZ*. Deletions of singular U1 snRNA gene significantly interfere with transcription and 3'-end mRNA formation. PLOS Genetics, 2023, 19(11): e1011021, PMID: 37917726.
5) Fan YJ# *, Ding Z#, Zhang Y#, Su R, Yue JL, Liang AM, Huang QW, Meng YR, Li M, Xue Y, and Xu YZ*. Sex-lethal regulates back-splicing and generation of the sex-differentially expressed circular RNAs. Nucleic Acids Research, 2023, 51(10): 5228-5241, PMID: 37070178.
6) Zhang B, Ding Z, Li L, Xie LK, Fan YJ, and Xu YZ*. Two oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in Drosophila. PLoS Genetics, 2021, 17(11): e1009861, PMID: 34723968.
7) Li L, Ding Z, Pang T-L, Zhang B, Li C-H, Liang A-M, Wang Y-R, Zhou Y, Fan YJ* and Xu YZ*. Defective Minor Spliceosomes Induce SMA-associated phenotypes through Sensitive Intron-Containing Neural Genes in Drosophila. Nature Communications, 2020, 11(1): 5608, PMID: 33154379. Recommended in the focus of “From Brain to Behaviour”.
8) Shao W, Ding Z, Zheng ZZ, Shen JJ, Shen YX, Pu J, Fan YJ, Query CC* and Xu YZ*. Prp5–Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription. Nucleic Acids Research, 2020, 48: 5799-5813, PMID: 32399566.
9) Lv M#, Yao Y#, Li F, Xu L, Yang L, Gong Q, Xu YZ, Shi Y, Fan YJ* and Tang Y*. Structural insights reveal the specific recognition of roX RNA by the dsRNA-binding domains of the RNA helicase MLE and its indispensable role in dosage compensation in Drosophila. Nucleic Acids Research, 2019, 47(6): 3142-3157, PMID: 30649456.
10) Qiu C#, Zhang Y#, Fan YJ#, Pang TL, Su Y, Zhan S* and Xu YZ*. HITS-CLIP reveals sex-differential RNA binding and alterative splicing regulation of SRm160 in Drosophila. Journal of Molecular Cell Biology, 2019, 11(2): 170–181, PMID: 29750417.
11) Gao JL#, Fan YJ#, Wang XY#, Zhang Y, Pu J, Li L, Shao W, Zhan S, Hao J and Xu YZ*. A conserved intronic U1 snRNP-binding sequence promotes trans-splicing in Drosophila. Genes & Development, 2015, 29(7):760-771, PMID: 25838544.
12) Fan YJ*, Gittis AH, Juge F, Qiu C, Xu YZ and Rabinow L*. Multifunctional RNA processing protein SRm160 induces apoptosis and regulates eye and genital development in Drosophila, Genetics, 2014, 197(4): 1251-1265, PMID: 24907259.
13) Fan YJ, Schlierf M, Gaspar AC, Dreux C, Kpebe A, Chaney L, Mathieu A, Hitte C, Grémy O, Sarot E, Horn M, Zhao YL, Kinzy TG and Rabinow L. Drosophila translational elongation factor-1gamma is modified in response to DOA kinase activity and is essential for cellular viability, Genetics, 2010, 184(1):141-154, PMID: 19841092.
