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Xinghua Zhang, PhD
Professor
College of Life Sciences, Wuhan University
Wuhan 430072, China
Email: zhxh@whu.edu.cn
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Education
2004.9 – 2009.7 Ph.D.
Biophysics and Soft Matter, Institute of Physics, Chinese Academy of Sciences
2000.9 – 2004.7 B.S.
Physics, Shandong University, Jinan, China.
Work Experience
2015 – Present: Professor, College of Life Sciences, Wuhan University, Wuhan, China.
2010 – 2015: Research Scientist, Singapore-MIT Alliance for Research and Technology, Singapore
2009 – 2010: Patent examiner, State Intellectual Property Office of China, Beijing, China
Research Interests
Single-molecule techniques, DNA and RNA structural dynamics, DNA probe applications
Selected Publications
As the corresponding author:
1. Yuru Hu#, Hongyun Li#*, Chen Zhang#, Jingjing Feng#, Wenxu Wang, Wei Chen, Miao Yu, Xinghua Zhang*, Zheng Liu*, "DNA-based ForceChrono Probes for Deciphering Single-Molecule Force Dynamics in Living Cells", Cell, in press (2024)
2. Peng Chen#, Jin Zhou#, Huan Liu#, Erchi Zhou#, Boxiao He, Yankang Wu, Hongjian Wang, Zaiqiao Sun, Chonil Paek, Jun Lei, Yongshun Chen*, Xinghua Zhang*, Lei Yin*, “Engineering of Cas12a nuclease variants with enhanced genome-editing specificity”, PLoS Biology, 22(3): e3002514 (2024)
3. Hai-Long Dong#, Chen Zhang#, Liang Dai, Yan Zhang*, Xing-Hua Zhang*, and Zhi-Jie Tan*, “The origin of different bending stiffness between double-stranded RNA and DNA revealed by magnetic tweezers and simulations.”, Nucleic Acids Research, gkae063 (2024)
4. Ya-Jun Yang, Hang Fu, Xiao-Lu Li, Hong-Yu Yang, Er-Chi Zhou, Cheng-Yu Xie, Shu-Wen Wu, Fan He*, Yan Zhang* and Xing-Hua Zhang*, "A mutation-sensitive, multiplexed and amplification-free detection of nucleic acids by stretching single-molecule tandem hairpin probes.", Nucleic Acids Research, 51(17): e90 (2023)
5. Fu-Jia Tian#, Chen Zhang #, Erchi Zhou#, Hai-Long Dong, Zhi-Jie Tan, Xing-Hua Zhang* and Liang Dai*，"Universality in RNA and DNA deformations induced by salt, temperature change, stretching force, and protein binding." PNAS, 120 (20) e2218425120 (2023)
6. Xiao-Cong Zhao#, Hai-Long Dong#, Xiao-Lu Li, Hong-Yu Yang, Xue-Feng Chen, Liang Dai, Wen-Qiang Wu, Zhi-Jie Tan* and Xing-Hua Zhang*, “5-methyl-cytosine stabilizes DNA but hinders DNA hybridization revealed by magnetic tweezers and simulations.” Nucleic Acids Research, 50 (21): 12344–12354 (2022)
7. Chen Zhang#, Fujia Tian#, Ying Lu, Bing Yuan, Zhi-Jie Tan, Xing-Hua Zhang* and Liang Dai*, “Twist-diameter coupling drives DNA twist changes with salt and temperature.” Science Advances, 8(12), abn1384 (2022)
8. Xiao-Wei Qiang#, Chen Zhang#, Hai-Long Dong#, Fu-Jia Tian, Hang Fu, Ya-Jun Yang, Liang Dai, Xing-Hua Zhang*, Zhi-Jie Tan*, “Multivalent cations reverse the twist-stretch coupling of RNA.” Physical Review Letters, 126, 108103. (2022)
9. Xuejie Wang#, Yang Dong#, Xiaocong Zhao#, Jinbao Li, Jordan Lee, Zhenxin Yan, Shuangshuang Yang, Wenqiang Wu, Ximiao Hou, Guangxue Liu, Yueyue Zhang, Lun Song, Gang Cai, Qing Li, Grzegorz Ira, Xinghua Zhang*, Xuefeng Chen*, “Rtt105 promotes high-fidelity DNA replication and repair by regulating the single-stranded DNA binding factor RPA.” PNAS, 118 (25): e2106393118 (2021)
10. Ya-Jun Yang, Hai-Long Dong, Xiao-Wei Qiang, Hang Fu, Er-Chi Zhou, Chen Zhang, Lei Yin, Xue-Feng Chen, Fu-Chao Jia, Liang Dai, Zhi-Jie Tan, and Xing-Hua Zhang*. “Cytosine methylation enhances DNA condensation revealed by equilibrium measurements using magnetic tweezers”. Journal of the American Chemical Society. 142 (20): 9203–9209 (2020)
11. Hang Fu#, Chen Zhang#, Xiao-Wei Qiang#, Ya-Jun Yang, Zhi-Jie Tan* and Xing-Hua Zhang*. “Opposite effects of high-valent cations on the elasticities of DNA and RNA duplexes revealed by magnetic tweezers.” Physical Review Letters. 124(5): 058101 (2020)
12. Chen Zhang#, Hang Fu#, Yajun Yang#, Erchi Zhou, Zhijie Tan, Huijuan You, Xinghua Zhang*. “The Mechanical Properties of RNA-DNA Hybrid Duplex Stretched by Magnetic Tweezers”. Biophysical Journal. 116 (2): 196-204 (2019) (Selected as the best paper of 2019)
13. Ya-Jun Yang#, Lun Song, Xiao-Cong Zhao, Chen Zhang, Wen-Qiang Wu, Hui-Juan You, Hang Fu, Er-Chi Zhou, Xing-Hua Zhang*. “A Universal Assay for Making DNA, RNA, and RNA–DNA Hybrid Configurations for Single-Molecule Manipulation in Two or Three Steps without Ligation”. ACS synthetic biology. 8(7): 1663-1672 (2019)
14. Ju-Hui Liu #, Kun Xi #, Xi Zhang, Lei Bao, Xing-Hua Zhang*, Zhi-Jie Tan*. “Structural Flexibility of DNA-RNA Hybrid Duplex: Stretching and Twist-Stretch Coupling”. Biophysical Journal. 117 (1): 74-86 (2019)
15. Xiao-Cong Zhao #, Hang Fu, Lun Song, Ya-Jun Yang, Er-Chi Zhou, Guang-Xue Liu, Xue-Feng Chen, Zhuo Li, Wen-Qiang Wu, Xing-Hua Zhang*, “S‑DNA and RecA/RAD51-Mediated Strand Exchange in Vitro”. Biochemistry. 58 (15): 2009-2016 (2019)
As the first author:
1. Xinghua Zhang, Yuanyuan Qu, Hu Chen, Ioulia Rouzina, Shengli Zhang, Patrick S. Doyle*, and Jie Yan*, “Interconversion between three overstretched DNA structures.” Journal of the American Chemical Society, 136 (45): 16073-16080 (2014)
2. Xinghua Zhang, Hu Chen, Shimin Le, Ioulia Rouzina, Patrick S. Doyle*, and Jie Yan*, “Revealing the competition between peeled ssDNA, melting bubbles and S-DNA during DNA overstretching by single-molecule calorimetry”, PNAS, 110 (10): 3865-3870 (2013)
3. Xinghua Zhang, Hu Chen, Hongixa Fu, Patrick S. Doyle*, and Jie Yan*, "Two distinct overstretched DNA structures revealed by single-molecule thermodynamics measurements", PNAS, 109 (21): 8103-8108 (2012)
