Yi Liang
Prof. Yi Liang, Ph.D.
Laboratory of Structural Biology
State Key Laboratory of Virology
College of Life Sciences, Wuhan University
Wuhan 430072, China
Tel: +86-27-68754902
Fax: +86-27-68754902
Email: liangyi@whu.edu.cn
EDUCATION
B.S., Wuhan University, 1985
M.S., Wuhan University, 1988
Ph.D., Wuhan University, 1998, Thesis topic: Thermochemical Studies on Enzyme-Catalyzed Reactions and the Cleavage of DNA, with Prof. S. S. Qu on Biophysical Chemistry
Postdoctoral Fellow, Institute of Biophysics, the Chinese Academy of Sciences, 1998-2000, with Prof. C. L. Tsou on Protein Folding
POSITIONS
2001-2002: Invited Professor, Faculté de Pharmacie, Université Louis Pasteur, France, with Prof. Jacques Haiech on Proteomics
2001.12-Present: Full Professor of Biochemistry, Department of Biotechnology, College of Life Sciences, Wuhan University
2006-2007: Invited Professor, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, USA, with Prof. Allen P. Minton on Protein Folding
RESEACH INTERESTS
1. Mechanisms of protein folding and amyloid fibril formation in vitro and in vivo.
2. Mechanisms of signal transduction through protein-protein interaction network and thereby high-throughput drug screening.
3. New drug design based on protein misfolding and protein interactions.
TEACHING EXPERIENCE
Course: Structural Biology (for undergraduate students and graduated students)
SELECTED PUBLICATIONS
1. Zhong-Ying Mo, Ying-Zhu Zhu, Hai-Li Zhu, Jun-Bao Fan, Jie Chen, Yi Liang* (2009) Low micromolar zinc accelerates the fibrillization of human Tau via bridging Cys-291 and Cys-322. J. Biol. Chem. 284, 34648-34657.
2. Zheng Zhou, Jun-Bao Fan, Hai-Li Zhu, Frank Shewmaker, Xu Yan, Xi Chen, Jie Chen, Geng-Fu Xiao, Lin Guo, Yi Liang* (2009) Crowded cell-like environment accelerates the nucleation step of amyloidogenic protein misfolding. J. Biol. Chem. 284, 30148-30158.
3. Bing-Rui Zhou, Yi Liang*, Fen Du, Zheng Zhou, Jie Chen. (2004) Mixed macromolecular crowding accelerates the oxidative refolding of reduced, denatured lysozyme: Implications for protein folding in intracellular environments. J. Biol. Chem. 279, 55109-55116.
4. Yi Liang*, Fen Du, Sarah Sanglier, Bing-Rui Zhou, Yi Xia, Alain Van Dorsselaer, Clarisse Maechling, Marie-Claude Kilhoffer, Jacques Haiech. (2003) Unfolding of rabbit muscle creatine kinase induced by acid. A study using electrospray ionization mass spectrometry, isothermal titration calorimetry, and fluorescence spectroscopy. J. Biol. Chem. 278, 30098-30105.
5. Fen Du, Zheng Zhou, Zhong-Ying Mo, Jun-Zhi Shi, Jie Chen, Yi Liang*. (2006) Mixed macromolecular crowding accelerates the refolding of rabbit muscle creatine kinase: Implications for protein folding in physiological environments. J. Mol. Biol., 364, 469-482.
6. Fang Yang (Jr.), Min Zhang, Bing-Rui Zhou, Jie Chen, Yi Liang*. (2006) Oleic acid inhibits amyloid formation of the intermediate of alpha-lactalbumin at moderately acidic pH. J. Mol. Biol. 362, 821-834.
7. Yu-Ling Zhou, Jun-Ming Liao, Jie Chen, Yi Liang*. (2006) Macromolecular crowding enhances the binding of superoxide dismutase to xanthine oxidase: Implications for protein-protein interactions in intracellular environments. Int. J. Biochem. Cell Biol. 38, 1986-1994.
8. Hong Xu, Yi Liang*, Peng Zhang, Fen Du, Bing-Rui Zhou, Jun Wu, Jian-Hong Liu, Zhi-Gang Liu, Liang-Nian Ji. (2005) Biophysical studies of a ruthenium(II) polypyridyl complex binding to DNA and RNA prove that nucleic acid structure has significant effects on binding behaviors. J. Biol. Inorg. Chem. 10, 529-538.
9. Jun Wu, Fen Du, Peng Zhang, Izhar Ahmed Khan, Jie Chen, Yi Liang*. (2005) Thermodynamics of the interaction of aluminum ions with DNA: Implications for the biological function of aluminum. J. Inorg. Biochem. 99, 1145-1154.
10. Min Zhang, Fang Yang (Jr.), Fang Yang, Jie Chen, Cong-Yi Zheng, Yi Liang* (2009) Cytotoxic aggregates of alpha-lactalbumin induced by unsaturated fatty acid induce apoptosis in tumor cells. Chem.-Biol. Interact. 180, 131-142.
11. Jun-Ming Liao, Zhong-Ying Mo, Ling-Jia Wu, Jie Chen, Yi Liang*. (2008) Binding of calcium ions to Ras promotes Ras guanine nucleotide exchange under emulated physiological conditions. Biochim. Biophys. Acta 1784, 1560-1569.
12. Bing-Rui Zhou, Zheng Zhou, Qing-Lian Hu, Jie Chen, Yi Liang*. (2008) Mixed macromolecular crowding inhibits amyloid formation of hen egg white lysozyme. Biochim. Biophys. Acta 1784, 472-480.
13. Fang Yang (Jr.), Min Zhang, Jie Chen, Yi Liang*. (2006) Structural changes of alpha-lactalbumin induced by low pH and oleic acid. Biochim. Biophys. Acta 1764, 1389-1396.
14. Nan Chen, Jun-Bao Fan, Jin Xiang, Jie Chen, Yi Liang*. (2006) Enzymatic hydrolysis of microcrystalline cellulose in reverse micelles. Biochim. Biophys. Acta 1764, 1029-1035.
15. Fang Yang, Bing-Rui Zhou, Peng Zhang, Yan-Feng Zhao, Jie Chen, Yi Liang*. (2007) Binding of ferulic acid to cytochrome c enhances stability of the protein at physiological pH and inhibits cytochrome c-induced apoptosis. Chem.-Biol. Interact. 170, 231-243.
* Corresponding author.
RESEACH PROJECTS
1. Quality Control in Protein Biosynthesis, which is supported by National Key Basic Research Foundation of China (Grant 2006CB910301, 2006-2010).
2. Mechanisms of Prion Protein Amyloid Formation and Aggregation under emulated physiological conditions, which is supported by National Natural Science Foundation of China (Grant 30970599, 2010-2012).
3. Mechanisms of Lysozyme Amyloid Formation and Aggregation under Conditions of Macromolecular Crowding, which is supported by National Natural Science Foundation of China (Grant 30770421, 2008-2010).
4. Studies on the Interactions of G Protein Coupled Receptor Kinases with Calmodulin and the Related Regulatory Mechanism, which is supported by Program for New Century Excellent Talents in University from the Ministry of Education of China (Grant NCET-04-0670, 2005-2008).
5. Fundamental Information for the Interactions of Calmodulin with Target Proteins and the Related Regulatory Mechanism, which is supported by National Natural Science Foundation of China (Grant 90408012, 2005-2007).
6. Application of Microfluidic Chip on Screening of Drugs for G Protein Coupled Receptor-Related Diseases, which is supported by Project 863 from the Ministry of Science and Technology of China (Grant 2004AA404260, 2004-2005).
7. Mechanisms of Protein Interactions under Conditions of Macromolecular Crowding, which is supported by National Natural Science Foundation of China (Grant 30370309, 2004-2006).
8. Mechanism of rhodium(III) complexes interacting with RNA, which is supported by National Natural Science Foundation of China (Grant 30570421, 2006-2008).
9. Microcalorimetric Studies on the Mechanism of Protein Folding Using Monoclonal Antibodies as Probes, which was supported by National Natural Science Foundation of China (Grant 39970164, 2000-2002) and K. C. Wong Education Foundation, Hong Kong.
10. Microcalorimetric Studies on the Mechanism of Molecular Chaperone-assisted Protein Folding, which is supported by Start-up Program from Wuhan University (Grant No. 00029, 2001-2005).
SCIENTIFIC AWAEDS
1. An Award of Natural Science, Second Class, of Hubei Province in China for “Studies on the Basic Theories of Chemical Reaction Engineering and Engineering Application of Multiphase Reactors” (in 2002).
NATIONAL SERVICE APPOINTMENT
2006-Present: Committee member of the Chinese Society of Biochemistry and Molecular Biology-Protein branch (Chinese Protein Society).
2006-Present: Committee member of the Chinese Society of Biochemistry and Molecular Biology-Enzymology branch.
2007-Present: Member of the editorial board of Acta Biochimica et Biophysica Sinica
Yi Liang
Prof. Yi Liang, Ph.D.
Laboratory of Structural Biology
State Key Laboratory of Virology
College of Life Sciences, Wuhan University
Wuhan 430072, China
Tel: +86-27-68754902
Fax: +86-27-68754902
Email: liangyi@whu.edu.cn
EDUCATION
B.S., Wuhan University, 1985
M.S., Wuhan University, 1988
Ph.D., Wuhan University, 1998, Thesis topic: Thermochemical Studies on Enzyme-Catalyzed Reactions and the Cleavage of DNA, with Prof. S. S. Qu on Biophysical Chemistry
Postdoctoral Fellow, Institute of Biophysics, the Chinese Academy of Sciences, 1998-2000, with Prof. C. L. Tsou on Protein Folding
POSITIONS
2001-2002: Invited Professor, Faculté de Pharmacie, Université Louis Pasteur, France, with Prof. Jacques Haiech on Proteomics
2001.12-Present: Full Professor of Biochemistry, Department of Biotechnology, College of Life Sciences, Wuhan University
2006-2007: Invited Professor, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, USA, with Prof. Allen P. Minton on Protein Folding
RESEACH INTERESTS
1. Mechanisms of protein folding and amyloid fibril formation in vitro and in vivo.
2. Mechanisms of signal transduction through protein-protein interaction network and thereby high-throughput drug screening.
3. New drug design based on protein misfolding and protein interactions.
TEACHING EXPERIENCE
Course: Structural Biology (for undergraduate students and graduated students)
SELECTED PUBLICATIONS
1. Zhong-Ying Mo, Ying-Zhu Zhu, Hai-Li Zhu, Jun-Bao Fan, Jie Chen, Yi Liang* (2009) Low micromolar zinc accelerates the fibrillization of human Tau via bridging Cys-291 and Cys-322. J. Biol. Chem. 284, 34648-34657.
2. Zheng Zhou, Jun-Bao Fan, Hai-Li Zhu, Frank Shewmaker, Xu Yan, Xi Chen, Jie Chen, Geng-Fu Xiao, Lin Guo, Yi Liang* (2009) Crowded cell-like environment accelerates the nucleation step of amyloidogenic protein misfolding. J. Biol. Chem. 284, 30148-30158.
3. Bing-Rui Zhou, Yi Liang*, Fen Du, Zheng Zhou, Jie Chen. (2004) Mixed macromolecular crowding accelerates the oxidative refolding of reduced, denatured lysozyme: Implications for protein folding in intracellular environments. J. Biol. Chem. 279, 55109-55116.
4. Yi Liang*, Fen Du, Sarah Sanglier, Bing-Rui Zhou, Yi Xia, Alain Van Dorsselaer, Clarisse Maechling, Marie-Claude Kilhoffer, Jacques Haiech. (2003) Unfolding of rabbit muscle creatine kinase induced by acid. A study using electrospray ionization mass spectrometry, isothermal titration calorimetry, and fluorescence spectroscopy. J. Biol. Chem. 278, 30098-30105.
5. Fen Du, Zheng Zhou, Zhong-Ying Mo, Jun-Zhi Shi, Jie Chen, Yi Liang*. (2006) Mixed macromolecular crowding accelerates the refolding of rabbit muscle creatine kinase: Implications for protein folding in physiological environments. J. Mol. Biol., 364, 469-482.
6. Fang Yang (Jr.), Min Zhang, Bing-Rui Zhou, Jie Chen, Yi Liang*. (2006) Oleic acid inhibits amyloid formation of the intermediate of alpha-lactalbumin at moderately acidic pH. J. Mol. Biol. 362, 821-834.
7. Yu-Ling Zhou, Jun-Ming Liao, Jie Chen, Yi Liang*. (2006) Macromolecular crowding enhances the binding of superoxide dismutase to xanthine oxidase: Implications for protein-protein interactions in intracellular environments. Int. J. Biochem. Cell Biol. 38, 1986-1994.
8. Hong Xu, Yi Liang*, Peng Zhang, Fen Du, Bing-Rui Zhou, Jun Wu, Jian-Hong Liu, Zhi-Gang Liu, Liang-Nian Ji. (2005) Biophysical studies of a ruthenium(II) polypyridyl complex binding to DNA and RNA prove that nucleic acid structure has significant effects on binding behaviors. J. Biol. Inorg. Chem. 10, 529-538.
9. Jun Wu, Fen Du, Peng Zhang, Izhar Ahmed Khan, Jie Chen, Yi Liang*. (2005) Thermodynamics of the interaction of aluminum ions with DNA: Implications for the biological function of aluminum. J. Inorg. Biochem. 99, 1145-1154.
10. Min Zhang, Fang Yang (Jr.), Fang Yang, Jie Chen, Cong-Yi Zheng, Yi Liang* (2009) Cytotoxic aggregates of alpha-lactalbumin induced by unsaturated fatty acid induce apoptosis in tumor cells. Chem.-Biol. Interact. 180, 131-142.
11. Jun-Ming Liao, Zhong-Ying Mo, Ling-Jia Wu, Jie Chen, Yi Liang*. (2008) Binding of calcium ions to Ras promotes Ras guanine nucleotide exchange under emulated physiological conditions. Biochim. Biophys. Acta 1784, 1560-1569.
12. Bing-Rui Zhou, Zheng Zhou, Qing-Lian Hu, Jie Chen, Yi Liang*. (2008) Mixed macromolecular crowding inhibits amyloid formation of hen egg white lysozyme. Biochim. Biophys. Acta 1784, 472-480.
13. Fang Yang (Jr.), Min Zhang, Jie Chen, Yi Liang*. (2006) Structural changes of alpha-lactalbumin induced by low pH and oleic acid. Biochim. Biophys. Acta 1764, 1389-1396.
14. Nan Chen, Jun-Bao Fan, Jin Xiang, Jie Chen, Yi Liang*. (2006) Enzymatic hydrolysis of microcrystalline cellulose in reverse micelles. Biochim. Biophys. Acta 1764, 1029-1035.
15. Fang Yang, Bing-Rui Zhou, Peng Zhang, Yan-Feng Zhao, Jie Chen, Yi Liang*. (2007) Binding of ferulic acid to cytochrome c enhances stability of the protein at physiological pH and inhibits cytochrome c-induced apoptosis. Chem.-Biol. Interact. 170, 231-243.
* Corresponding author.
RESEACH PROJECTS
1. Quality Control in Protein Biosynthesis, which is supported by National Key Basic Research Foundation of China (Grant 2006CB910301, 2006-2010).
2. Mechanisms of Prion Protein Amyloid Formation and Aggregation under emulated physiological conditions, which is supported by National Natural Science Foundation of China (Grant 30970599, 2010-2012).
3. Mechanisms of Lysozyme Amyloid Formation and Aggregation under Conditions of Macromolecular Crowding, which is supported by National Natural Science Foundation of China (Grant 30770421, 2008-2010).
4. Studies on the Interactions of G Protein Coupled Receptor Kinases with Calmodulin and the Related Regulatory Mechanism, which is supported by Program for New Century Excellent Talents in University from the Ministry of Education of China (Grant NCET-04-0670, 2005-2008).
5. Fundamental Information for the Interactions of Calmodulin with Target Proteins and the Related Regulatory Mechanism, which is supported by National Natural Science Foundation of China (Grant 90408012, 2005-2007).
6. Application of Microfluidic Chip on Screening of Drugs for G Protein Coupled Receptor-Related Diseases, which is supported by Project 863 from the Ministry of Science and Technology of China (Grant 2004AA404260, 2004-2005).
7. Mechanisms of Protein Interactions under Conditions of Macromolecular Crowding, which is supported by National Natural Science Foundation of China (Grant 30370309, 2004-2006).
8. Mechanism of rhodium(III) complexes interacting with RNA, which is supported by National Natural Science Foundation of China (Grant 30570421, 2006-2008).
9. Microcalorimetric Studies on the Mechanism of Protein Folding Using Monoclonal Antibodies as Probes, which was supported by National Natural Science Foundation of China (Grant 39970164, 2000-2002) and K. C. Wong Education Foundation, Hong Kong.
10. Microcalorimetric Studies on the Mechanism of Molecular Chaperone-assisted Protein Folding, which is supported by Start-up Program from Wuhan University (Grant No. 00029, 2001-2005).
SCIENTIFIC AWAEDS
1. An Award of Natural Science, Second Class, of Hubei Province in China for “Studies on the Basic Theories of Chemical Reaction Engineering and Engineering Application of Multiphase Reactors” (in 2002).
NATIONAL SERVICE APPOINTMENT
2006-Present: Committee member of the Chinese Society of Biochemistry and Molecular Biology-Protein branch (Chinese Protein Society).
2006-Present: Committee member of the Chinese Society of Biochemistry and Molecular Biology-Enzymology branch.
2007-Present: Member of the editorial board of Acta Biochimica et Biophysica Sinica (ABBS).
