学习经历

2007.8 — 2013.12 美国堪萨斯大学医学中心，微生物学博士

2003.9 — 2007.6 武汉大学生命科学学院生命科学与技术基地班, 生物学学士

主要工作经历与任职情况

2019.6 — 至今　　武汉大学生命科学学院特聘研究员

2018.7 — 2019.6　堪萨斯大学医学中心研究助理教授

2014.2 — 2018.6　堪萨斯大学医学中心博士后

主要社会兼职

2021至今 微生物学通报编委

2021至今 Frontiers in Microbiology Guest Associate Editor

2021 Frontiers in Microbiology Research Topic “ Regulation of Prokaryotic Cell Division” Lead Guest Editor

PNAS, EMBO Report, Front Microbiol 和Cell Biosci等杂志应邀审稿人

承担课程

本科生：微生物学、微生物学实验

研究生：生命科学前沿进展

主要研究领域及兴趣

1. 细菌细胞生长和分裂的调控机制。细胞的生长和分裂是所有细菌增殖的必要过程。杆状细菌如大肠杆菌的生长和分裂分别由蛋白复合体elongasome 和divisome介导完成。Elongasome和 divisome分别在细胞侧壁和分裂位点合成细胞壁，从而控制细胞的生长和分裂。目前，这两个蛋白复合体的大部分组分蛋白都已经被发现，但是这些蛋白如何组装成elongasome 和divisome以及如何调控细胞壁的合成尚不清楚。因此，本实验室将通过遗传学、生物化学以及细胞生物学等一系列手段研究elongasome和divisome的组装和工作机理，加深我们对细菌基本生命活动的了解。

2. 细胞壁的合成和抗生素的研发。由于抗生素的滥用和过度使用，很多细菌都对抗生素产生了抗药性和耐药性。细菌的抗药性和耐药性问题已经成为人类公共卫生最大的威胁，我们迫切需要研发新的抗生素。抑制细胞壁合成是杀死细菌最有效的途径之一，而elongasome和divisome 是细菌细胞壁合成最重要的蛋白复合物，抑制它们的活性可以阻断细胞壁合成从而可以杀死细菌。本实验室将研究elongasome和dvisome合成细胞壁的机理，同时以复合体中的重要蛋白为靶点，研发新的抗菌药物。

主持课题项目

(1)　国家自然科学基金面上项目, 32270049, 嗜盐古菌细胞分裂机制的研究, 2023-01 至 2026-12, 主持

(2)　国家自然科学基金面上项目, 32070032, 分裂蛋白复合体FtsEX调控细菌细胞分裂的机制, 2021-01 至 2024-12, 主持

(3)　武汉大学引进人才启动经费，2019-06至2025-05，主持

(4)　中央高校拔尖创新人才资助项目，2021-01至2022-12,主持

发表的论文（#并列第一作者，*通讯作者）

1. Yan-Ran Xu, Meng-Ling Shi, Yu Zhang , Na Kong, Cong Wang, Yi-Feng Xiao, Shi-Shen Du, Qi-Yun Zhu, Cao-Qi Lei*. (2022) Tankyrases inhibit innate antiviral response by PARylating VISA/MAVS and priming it for RNF146-mediated ubiquitination and degradation. PNAS 119(26):e2122805119

2. Ying Li, Adrien Boes, Yuanyuan Cui, Shan Zhao, Qingzhen Liao, Han Gong, Eefjan Breukink, Joe Lutkenhaus, Mohammed Terrak*, Shishen Du*. (2022) Identification of the potential active site of the septal peptidoglycan polymerase FtsW. PLoS Genet 18(1):e1009993

3. Kyung-Tae Park, Sebastien Pichoff, Shishen Du, Joe Lutkenhaus*. FtsA acts through FtsW to promote cell wall synthesis during cell division in Escherichia coli. PNAS 118(35):e2107210118

4. Mart Krupovic, Dann Turner, Vera Morozova, Mike Dyall-Smith, Hanna M Oksanen, Rob Edwards, Bas E Dutilh, Susan M Lehman, Alejandro Reyes, Diana P Baquero, Matthew B Sullivan, Jumpei Uchiyama, Jesca Nakavuma, Jakub Barylski, Mark J Young, Shishen Du, Poliane Alfenas-Zerbini, Alla Kushkina, Andrew M Kropinski, Ipek Kurtböke, J Rodney Brister, Cédric Lood, B L Sarkar, Tong Yigang, Ying Liu, Li Huang, Johannes Wittmann, Nina Chanishvili, Leonardo J van Zyl, Janis Rumnieks, Tomohiro Mochizuki, Matti Jalasvuori, Ramy K Aziz, Małgorzata Łobocka, Kenneth M Stedman, Andrey N Shkoporov, Annika Gillis, Xu Peng, François Enault, Petar Knezevic, Rob Lavigne, Sung-Keun Rhee, Virginija Cvirkaite-Krupovic, Cristina Moraru, Andrea I Moreno Switt, Minna M Poranen, Andrew Millard, David Prangishvili, Evelien M Adriaenssens. (2021) Bacterial Viruses Subcommittee and Archaeal Viruses Subcommittee of the ICTV: update of taxonomy changes in 2021. Arch Virol 166(11):3239-3244

5. Ying Li#, Han Gong, Rui Zhan, Shushan Ouyang, Kyung-Tae Park, Joe Lutkenhaus*, and Shishen Du*.  (2021) Genetic analysis of the septal peptidoglycan synthase FtsWI complex supports a conserved activation mechanism for SEDS-bPBP complexes. PLoS Genet 17(4):e1009366

6. Kyung-Tae Park, Shishen Du, and Joe Lutkenhaus. (2020) Essential Role for FtsL in Activation of Septal Peptidoglycan Synthesis. mBio 11(6):e03012-20

7. Beibei Chen, Zhao Chen, Yuchen Wang, Han Gong, Jiao Wang, Shushan Ouyang, Wenqiang Guan, Mart Krupovic, Xiangdong Chen*, and Shishen Du*. (2020) ORF4 of the temperate archaeal virus SNJ1 governs the lysis-lysogeny switch and superinfection immunity. J Virol 94(16):e00841-20

8. Shishen Du*, Sebastien Pichoff, and Joe Lutkenhaus*. (2020) Roles of ATP hydrolysis by FtsEX and interaction with FtsA in regulation of septal peptidoglycan synthesis and hydrolysis. mBio 11(4):e01247-20

9. Sebastien Pichoff, Shishen Du, Joe Lutkenhaus*. (2019) Roles of FtsEX in cell division. Res Microbiol S0923-2508(19)30082-8

10. Shishen Du, Wyatt Henke, Sebastien Pichoff, Joe Lutkenhaus*. (2019) How FtsEX localizes to the Z ring and interacts with FtsA to regulate cell division. Mol Microbiol 112(3):881-895

11. Shishen Du, Joe Lutkenhaus*. (2019) At the heart of bacterial cytokinesis: the Z ring.  Trends Microbiol S0966-842X(19)30122-2

12. Qinggong Guo, Beibei Chen, Yishuai Tu, Shishen Du*, Xiangdong Chen*. (2019) Prophage LambdaSo uses replication interference to suppress reproduction of co-existing temperate phage MuSo2 in Shewanella oneidensis MR-1. Environ Microbiol 21(6)2079-2094

13. Yunqian Qiao, Jiao Wang, He Wang, Baozhong Chai, Chufeng Rao, Xiangdong Chen*, Shishen Du*. (2018) 4-hydroxyphenylpyruvate dioxygenase thermolability is responsible for temperature-dependent melanogenesis in Aeromonas Salmonicida subsp. Salmonicida. Appl Environ Microbiol 13; 85 (1). pii::e01926-18

14. Shishen Du, Sebastien Pichoff, Kruse K, Joe Lutkenahus*. (2018) FtsZ filaments have the opposite kinetic polarity of microtubules.  Proc Natl Acad Sci USA 115(42):10768-10773 (Faculty of 1000)

15. Sebastien Pichoff#, Shishen Du#, Joe Lutkenhaus*. (2018) Disruption of divisome assembly rescued by FtsN-FtsA interaction in E. coli. Proc Natl Acad Sci USA 115(29):E6855-E6862 (#:equal contribution)

16. Kyung-Tae Park, Alex Dajkovic, Mark Wissel, Shishen Du, Joe Lutkenhaus*. (2018) MinC and FtsZ mutant analysis provides insight into MinC/MinD-mediated Z Ring disassembly.  J Biol Chem. 293(16)::5834-5846

17. Joe Lutkenhaus*, Shishen Du. (2017) E. coli Cell cycle Machinery. Subcell Biochem 84: 27-65

18. Shishen Du, Joe Lutkenhaus*. (2017) The N-succinyl-ll-diaminopimelic acid desuccinylase acts through ZapB to promote septum formation in Escherichia coli. Mol Microbiol 105(2):326-345 (Faculty 1000)

19. Shishen Du, Joe Lutkenhaus*. (2017) Assembly and activation of the Escherichia coli divisome. Mol Microbiol 105(2):177-187

20. Shishen Du, Sebastien Pichoff, Joe Lutkenhaus*. (2016) FtsEX acts on FtsA to regulate divisome assembly and activation in E. coli. Proc Natl Acad Sci USA 113 (34):E5052-61(Faculty 1000)

21. Kyung-Tae Park, Shishen Du, Joe Lutkenhaus*. (2015) MinC/MinD copolymers are not required for Min function. Mol Microbiol 98 (5): 895-909

22. Sebastien Pichoff, Shishen Du, Joe Lutkenhaus*. (2015) The bypass of ZipA by overexpression of FtsN requires a previously unknown conserved FtsN motif essential for FtsA-FtsN interaction supporting a model in which FtsA monomers recruit late cell division proteins to the Z ring. Mol Microbiol  95 (6): 971-987

23. Shishen Du, Kyung-Tae Park, Joe Lutkenhaus*. (2015) Oligomerization of FtsZ converts the FtsZ tail motif (Conserved carboxy-terminal peptide) into a multivalent ligand with high avidity for partners ZipA and SlmA. Mol Microbiol 95 (2): 173-188

24. Shishen Du, Joe Lutkenhaus*. (2014) SlmA antagonism of FtsZ ring assembly employs a two-pronged mechanism like MinCD. PloS Genetics 10.1371-e1004460

25. Joe Lutkenhaus*, Sebastien Pichoff, Shishen Du. (2012) Bacterial cytokinesis: From Z ring to divisome. Cytoskeleton (Hoboken) 69: 778-790

26.   Shishen Du, Joe Lutkenhaus*. (2012) MipZ: One for the Pole, Two for the DNA. Molecular Cell 46: 239-240

招生招聘

欢迎对微生物遗传和生理、细胞生物学以及抗生素耐药性感兴趣的本科生、研究生或者博士后加入我们的实验室。