学习经历
1985.9-1989.6： 武汉大学生物系微生物专业 学士
1989.9-1992.6： 武汉大学生物系微生物专业 硕士
1992.9-1995.6： 中国科学院微生物研究所酶室生物化学专业 博士
工作经历
1995.7-1997.9： 武汉大学生命科学学院 讲师
1997.10-2003.10： 武汉大学生命科学学院 副教授
2000.9-2002.9： 日本国立食品研究所酶室 博士后
2003.10-现在： 武汉大学生命科学学院教授、博士生导师
2003-2006： 生物技术系副主任
2006-2015： 微生物系主任
2010-2020： 生科院副院长
社会兼职
中国微生物学会酶工程专业委员会委员
湖北省暨武汉市微生物学会常务理事
湖北省生物工程学会理事
《Applied and Environmental Microbiology》编委 (Editorial Board Member, 2019-2024)
承担课程
《大学生物学》（本科生课程）
《极端微生物》（硕士生课程）
研究方向
● 极端（嗜热、嗜盐等）微生物酶结构与功能、适应机理及定向分子进化
● 极端古菌蛋白质组及其噬菌体分子遗传学
● 微生物基因及酶资源开发和应用
研究概述
极端微生物生活在高温、低温、高盐、极端pH等各种极端环境中，它们所产生的酶（极端酶）在极端条件下保持结构完整性并正常发挥生物学功能，而一般生物的酶在这些极端条件下将丧失活性。极端微生物的原噬菌体基因组也编码耐受极端条件的功能蛋白。阐明极端酶适应极端条件的独特分子机制，不仅深化我们对极端微生物适应机理的认识，也有助于我们开发新型生物催化剂。本实验室主要研究超嗜热微生物、嗜热微生物及嗜盐微生物酶（如蛋白酶）的结构、功能、生理作用及适应性分子机制，并发掘它们在极端条件下降解角蛋白、胶原蛋白、朊蛋白等难降解物质方面的应用潜力。
科研项目
作为主持人获武汉市晨光计划、教育部高等学校青年骨干教师培养计划、湖北省杰出青年基金、973子课题、国家自然科学基金面上项目等资助。
● 国家自然科学基金. 超嗜热古菌蛋白酶Pyrolysin响应温度变化的分子机制
● 国家自然科学基金. N端前肽介导的重组丝氨酸蛋白酶在大肠杆菌中跨外膜转运
● 国家自然科学基金. 基于嗜热、嗜温及嗜冷菌来源的三种高度同源蛋白酶的温度适应性分子机制研究
● 国家自然科学基金. 高温放线菌休眠芽胞表面活性蛋白酶的性质及功能研究
● 国家自然科学基金. 嗜热细菌胞外蛋白酶WF146的成熟机制及其调控机制
● 国家自然科学基金. 嗜热脂肪芽胞杆菌WF146高温蛋白酶“冷适应”研究
发表论文
1. Luo H, Qu X, Deng X, He L, Wu Y, Liu Y, He D, Yin J, Wang B, Gan F, Tang B*, Tang XF* (2024) HtrAs are essential for the survival of the haloarchaeon Natrinema gari J7-2 in response to heat, high salinity, and toxic substances. Appl Environ Microbiol 90(2):e0204823
2. Wu Y, Zhang J, Wang B, Zhang Y, Li H, Liu Y, Yin J, He D, Luo H, Gan F, Tang B*, Tang XF* (2023) Dissecting the arginine and lysine biosynthetic pathways and their relationship in haloarchaeon Natrinema gari J7-2 via endogenous CRISPR-Cas system-based genome editing. Microbiol Spectr 11(4):e0028823.
3. Huang Q, Zhang K, Li Y, Gan F, Tang XF*, Tang B* (2022) Auto- and hetero-catalytic processing of the N-terminal propeptide promotes the C-terminal fibronectin type III domain-mediated dimerization of a thermostable Vpr-like protease. Appl Environ Microbiol 88(21):e0150322.
4. Mei S, Li M, Sun Y, Deng X, Chen N, Liu Y, Yin J, Luo H, Wu Y, He D, Gan F, Tang B*, Tang XF* (2022) Sec-dependent secretion of subtilase SptE in haloarchaea facilitates its proper folding and heterocatalytic processing by halolysin SptA extracellularly. Appl Environ Microbiol 88(8):e0024622.
5. Zhang K, Huang Q, Li Y, Liu L, Tang XF*, Tang B* (2022) Maturation process and characterization of a novel thermostable and halotolerant subtilisin-like protease with high collagenolytic activity but low gelatinolytic activity. Appl Environ Microbiol 88(3):e0218421.
6. Wang J, Hao C, Cao L, Yao Y, Ding Y, Yang Y, Tang XF*, Tang B* (2021) Enhancing extracellular production of recombinant proteins in Escherichia coli by co-expressing with a haloarchaeal protein containing a putative LolA-like domain. Appl Microbiol Biotechnol 105(11):4609-20.
7. Ding Y, Yang Y, Ren Y, Xia J, Liu F, Li Y, Tang XF, Tang B* (2020) Extracellular production, characterization, and engineering of a polyextremotolerant subtilisin-like protease from feather-degrading Thermoactinomyces vulgaris strain CDF. Front Microbiol 11:605771.
8. Li B, Liu F, Ren Y, Ding Y, Li Y, Tang XF, Tang B* (2019) Complete genome sequence of Thermoactinomyces vulgaris strain CDF, a thermophilic bacterium capable of degrading chicken feathers.Microbiol Resour Announc 8(28):e00530-19.
9. Li M, Yin J, Mei S, Wang X, Tang XF*, Tang B* (2018) Halolysin SptA, a serine protease, contributes to growth-phase transition of haloarchaeon Natrinema sp. J7-2, and its expression involves cooperative action of multiple cis-regulatory elements.Front Microbiol 9:1799.
10. 高晓威，唐晓峰，唐兵* (2017) 超嗜热古菌的ATP 非依赖型蛋白酶和肽酶研究进展，微生物学报，57(9):1373-82.
11. Zhu F, Yang X, Wu Y, Wang Y, Tang XF, Tang B* (2017) Release of an HtrA-like protease from the cell surface of thermophilic Brevibacillus sp. WF146 via substrate-induced autoprocessing of the N-terminal membrane anchor. Front Microbiol 8:481.
12. Gao X, Zeng J, Yi H, Zhang F, Tang B*, Tang XF* (2017) Four inserts within the catalytic domain confer extra stability and activity to hyperthermostable pyrolysin from Pyrococcus furiosus. Appl Environ Microbiol 83(5):e03228-16.
13. Liu F, Zhao ZS, Ren Y, Cheng G, Tang XF, Tang B* (2016) Autocatalytic activation of a thermostable glutamyl endopeptidase capable of hydrolyzing proteins at high temperatures.Appl Microbiol Biotechnol 100(24):10429-41.
14. Tang W, Wu Y, Li M, Wang J, Mei S, Tang B*, Tang XF* (2016) Alternative translation initiation of a haloarchaeal serine protease transcript containing two in-frame start codons. J Bacteriol 198(13):1892-901. (Spotlight article)
15. Meng D, Dai M, Xu BL, Zhao ZS, Liang X, Wang M, Tang XF, Tang B* (2016) Maturation of fibrinolytic bacillopeptidase F involves both hetero- and autocatalytic processes. Appl Environ Microbiol 82(1):318-27.
16. Xu BL, Dai M, Chen Y, Meng D, Wang Y, Fang N, Tang XF, Tang B* (2015) Improving the thermostability and activity of a thermophilic subtilase by incorporating structural elements of its psychrophilic counterpart.Appl Environ Microbiol 81(18):6302-13.
17. Wang L, Cheng G, Ren Y, Dai Z, Zhao ZS, Liu F, Li S, Wei Y, Xiong J, Tang XF, Tang B* (2015) Degradation of intact chicken feathers by Thermoactinomyces sp. CDF and characterization of its keratinolytic protease.Appl Microbiol Biotechnol 99(9): 3949-59.
18. Du X, Li M, Tang W, Zhang Y, Zhang L, Wang J, Li T, Tang B, Tang XF* (2015) Secretion of Tat-dependent halolysin SptA capable of autocatalytic activation and its relation to haloarchaeal growth. Mol Microbiol 96(3):548-65.
19. Ji X, Zhang C, Fang Y, Zhang Q, Lin L, Tang B, Wei Y* (2015) Isolation and characterization of glacier VMY22, a novel lytic cold-active bacteriophage of Bacillus cereus. Virol Sin 30(1):52-8.
20. Zhang Y, Wang M, Du X, Tang W, Zhang L, Li M, Wang J, Tang B, Tang XF* (2014) Chitin accelerates activation of a novel haloarchaeal serine protease that deproteinizes chitin-containing biomass. Appl Environ Microbiol 80(18):5698-708.
21. Zeng J, Gao X, Dai Z, Tang B, Tang XF* (2014) Effects of metal ions on stability and activity of hyperthermophilic pyrolysin and further stabilization of this enzyme by modification of a Ca2+-binding site. Appl Environ Microbiol 80(9):2763-72.
22. Feng J, Wang J, Zhang Y, Du X, Xu Z, Wu Y, Tang W, Li M, Tang B, Tang XF* (2014) Proteomic analysis of the secretome of haloarchaeon Natrinema sp. J7-2. J Proteome Res 13(3):1248-58.
23. Zhu H, Xu BL, Liang X, Yang YR, Tang XF, Tang B* (2013) Molecular basis for auto- and hetero-catalytic maturation of a thermostable subtilase from thermophilic Bacillus sp. WF146. J Biol Chem 288(48):34826-38.
24. Feng J, Liu B, Zhang Z, Ren Y, Li Y, Gan F, Huang Y, Chen X, Shen P, Wang L, Tang B*, Tang XF* (2012) The complete genome sequence of Natrinema sp. J7-2, a haloarchaeon capable of growth on synthetic media without amino acid supplements. PLoS ONE 7(7): e41621.
25. Dai Z, Fu H, Zhang Y, Zeng J, Tang B, Tang XF* (2012) Insights into the maturation of hyperthermophilic pyrolysin and the roles of its N-terminal propeptide and long C-terminal extension.Appl Environ Microbiol 78(12):4233-41.
26. Xu Z, Du X, Li T, Gan F, Tang B, Tang XF* (2011) Functional insight into the C-terminal extension of halolysin SptA from haloarchaeon Natrinema sp. J7. PLoS ONE 6(8): e23562.
27. Liang X, Bian Y, Tang XF, Xiao G, Tang B* (2010) Enhancement of keratinolytic activity of a thermophilic subtilase by improving its autolysis resistance and thermostability under reducing conditions.Appl Microbiol Biotechnol 87(3):999-1006.
28. Fang N, Zhong CQ, Liang X, Tang XF, Tang B* (2010) Improvement of extracellular production of a thermophilic subtilase expressed in Escherichia coli by random mutagenesis of its N-terminal propeptide. Appl Microbiol Biotechnol 85(5):1473-81.
29. Zhong CQ, Song S, Fang N, Liang X, Zhu H, Tang XF, Tang B* (2009) Improvement of low-temperature caseinolytic activity of a thermophilic subtilase by directed evolution and site-directed mutagenesis. Biotechnol Bioeng 104(5):862-70. (Spotlight article)
30. Cheng G, Zhao P, Tang XF, Tang B* (2009) Identification and characterization of a novel spore-associated subtilase from Thermoactinomyces sp. CDF.Microbiology (Reading) 55:3661-72.
31. Zhang H, Cui P, Lin L, Shen P, Tang B, Huang YP* (2009) Transcriptional analysis of the hsp70 gene in a haloarchaeon Natrinema sp. J7 under heat and cold stress. Extremophiles 13(4):669-78.
32. Yang YR, Zhu H, Fang N, Liang X, Zhong CQ, Tang XF, Shen P, Tang B* (2008) Cold-adapted maturation of thermophilic WF146 protease by mimicking the propeptide binding interactions of psychrophilic subtilisin S41. FEBS Lett 582(17):2620-26.
33. 石万良，钟传奇，唐兵，沈萍*（2007）极端嗜盐古生菌(Natrinema sp.) R625胞外嗜盐蛋白酶的纯化和性质研究，微生物学报，47(1):161-3.
34. Bian Y, Liang X, Fang N, Tang XF, Tang B*, Shen P, Peng Z (2006) The roles of surface loop insertions and disulfide bond in the stabilization of thermophilic WF146 protease. FEBS Lett 580(25):6007-14. (Faculty 1000 of Biology)
35. Shi W, Tang XF, Huang Y, Gan F, Tang B*, Shen P (2006) An extracellular halophilic protease SptA from a halophilic archaeon Natrinema sp. J7: gene cloning, expression and characterization. Extremophiles 10(6):599-606.
36. Wu J, Bian Y, Tang B*, Chen X, Shen P, Peng Z (2004) Cloning and analysis of WF146 protease, a novel thermophilic subtilisin-like protease with four inserted surface loops. FEMS Microbiol Lett 230(2):251-8.
37. 刘军，陈向东，戴玄，唐兵，彭珍荣*（2004）表面活性剂对嗜热脂肪芽孢杆菌产高温蛋白酶的影响，微生物学杂志，24(6):58-59.
38. 刘军，陈向东，戴玄，唐兵，彭珍荣*（2004）嗜热脂肪芽孢杆菌高温蛋白酶分解毛发角蛋白的研究，氨基酸和生物资源，26(3):52-4.
39. Tang B, Nirasawa S, Kitaoka M, Marie-Claire C, Hayashi K* (2003) General function of N-terminal propeptide on assisting protein folding and inhibiting catalytic based on observations with a chimeric thermolysin-like protease. Biochem Biophys Res Commun 301:1093-8.
40. Tang B, Nirasawa S, Kitaoka M, Hayashi K* (2002) The role of the N-terminal propeptide of the pro-aminopeptidase processing protease: refolding, processing and enzyme inhibition. Biochem Biophys Res Commun 296 78-84.
41. Tang B, Nirasawa S, Kitaoka M, Hayashi K* (2002) In vitro stepwise autoprocessing of the proform of pro-aminopeptidase processing protease from Aeromonas caviae T-64. Biochim Biophys Acta 1696:16-27.
42. 唐兵，周林峰，陈向东，戴玄，彭珍荣*（2000）嗜热脂肪芽孢杆菌高温蛋白酶的产生条件及酶学性质，微生物学报，40(2):188-92.
43. Wei C, Tang B, Zhang Y, Yang K* (1999) Oxidative refolding of recombinant prochymosin. Biochem J 340:345-51.
44. 唐兵，杨开宇*（1997）凝乳酶原Cys45-Cys50二硫键功能的研究，中国科学，27(1):14-20.
45. 戴玄，唐兵，陈向东，彭珍荣*（1997）产高温蛋白酶微生物菌种资源的研究，微生物学杂志，17(3):25-9.
46. Tang B, Zhang S, Yang K* (1994) Assisted refolding of recombinant prochymosin with the aid of protein disulfide isomerase. Biochem J 301:17-20.
47. 唐兵，彭珍荣*（1992）发酵法生产左旋多巴提取工艺的研究，氨基酸杂志, 54(2):1-4.
48. 唐兵，彭珍荣*（1991）嗜麦芽假单胞菌生产左旋多巴发酵条件的研究，氨基酸杂志, 52(4):1-3.
