学习经历
1996.10-2001.3 日本京都大学大学院工学研究科合成及生物化学专业 博士
1989.9 -1992.7 武汉大学生命科学学院微生物学专业 硕士
1985.9 -1989.7 武汉大学生物系微生物学专业 学士
工作经历
2003.11-现在 武汉大学生命科学学院微生物学系 副教授、教授
2001.4-2003.8 日本国立产业技术综合研究所生物情报解析研究中心博士后 (JSPS Fellow)
1992.7-1996.9 武汉大学生命科学学院微生物学系 助教、讲师
承担课程
1、微生物学，全英文，本科生
2、微生物的世界，本科生
3、高级微生物学（部分），研究生
教学获奖
1、2022年武汉大学第二届教师教学创新大赛一等奖（正高组）
2、2013年湖北省高等学校教学成果一等奖（排名二）
3、2008年国家级教学团队（微生物学系列课程教学团队主要成员）
4、2005年国家级教学成果二等奖（排名第三）
5、2005年湖北省高等学校教学成果一等奖（排名第三）
主持和参加课题
主持多项国家自然科学基金、教育部留学回国人员科研启动基金等，参加973项目子项目。主持武汉大学教改项目1项，参加多项国家级、省部级教改项目。主持：
● 国家自然科学基金，嗜盐古菌HtrA蛋白酶的生理功能及作用机制研究
● 国家自然科学基金，嗜盐古菌Tat及Sec信号肽在胞外蛋白酶折叠、成熟及转运过程中的作用差异性的分子机制
● 国家自然科学基金，嗜盐古菌胞外蛋白酶分泌机制的研究
● 国家自然科学基金，嗜盐古菌蛋白酶SptA的C端延伸区功能研究
● 国家自然科学基金，极端嗜热古菌蛋白酶Pyrolysin插入序列功能的研究
主要社会兼职
中国微生物学会微生物学教学工作委员会委员，《微生物学通报》编委，中国微生物学会会员，中国遗传学会会员，美国微生物学会会员。
发表的论文（*通讯作者）
1. Huang Q, Zhang K, Li Y, Gan F, Tang XF*, Tang B*. (2022) Auto- and hetero-catalytic processing of the N-terminal propeptide promotes the C-terminal fibronectin type III domain-mediated dimerization of a thermostable Vpr-like protease. Appl Environ Microbiol 88(21):e0150322.
2. Mei S, Li M, Sun Y, Deng X, Chen N, Liu Y, Yin J, Luo H, Wu Y, He D, Gan F, Tang B*, Tang XF*. (2022) Sec-dependent secretion of subtilase SptE in haloarchaea facilitates its proper folding and heterocatalytic processing by halolysin SptA extracellularly. Appl Environ Microbiol 88(8):e0024622.
3. Zhang K, Huang Q, Li Y, Liu L, Tang XF*, Tang B*. (2022) Maturation process and characterization of a novel thermostable and halotolerant subtilisin-like protease with high collagenolytic activity but low gelatinolytic activity. Appl Environ Microbiol 88(3):e02184-21.
4. Wang J, Hao C, Cao L, Yao Y, Ding Y, Yang Y, Tang XF*, Tang B* (2021) Enhancing extracellular production of recombinant proteins in Escherichia coli by co-expressing with a haloarchaeal protein containing a putative LolA-like domain. Appl Microbiol Biotechnol 105(11):4609-20.
5. Ding Y, Yang Y, Ren Y, Xia J, Liu F, Li Y, Tang XF*, Tang B* (2020). Extracellular production, characterization, and engineering of a polyextremotolerant subtilisin-like protease from feather-degrading Thermoactinomyces vulgaris strain CDF. Front Microbiol 11: 605771.
6. Li B, Liu F, Ren Y, Ding Y, Li Y, Tang XF, Tang B* (2019) Complete genome sequence of Thermoactinomyces vulgaris strain CDF, a thermophilic bacterium capable of degrading chicken feathers. Microbiol Resour Announc 8(28):e00530-19.
7. Li M, Yin J, Mei S, Wang X, Tang XF*,Tang B* (2018) Halolysin SptA, a serine protease, contributes to growth-phase transition of haloarchaeon Natrinema sp. J7-2, and its expression involves cooperative action of multiple cis-regulatory elements. Front Microbiol 9:1799.
8. Perveen S, Rashid N, Tang XF, Imanaka T, Papageorgiou AC* (2017) Anthranilate phosphoribosyltransferase from the hyperthermophilic archaeon Thermococcus kodakarensis shows maximum activity with zinc and forms a unique dimeric structure. FEBS Open Bio 7(8):1217-30.
9. Gao X, Zeng J, Yi H, Zhang F, Tang B*, Tang XF* (2017) Four inserts within the catalytic domain confer extra stability and activity to hyperthermostable pyrolysin from Pyrococcus furiosus. Appl Environ Microbiol 83(5).pii:e03228-16.
10. Zhu F, Yang X, Wu Y, Wang Y, Tang XF, Tang B* (2017) Release of an HtrA-like protease from the cell surface of thermophilic Brevibacillus sp. WF146 via substrate-induced autoprocessing of the N-terminal membrane anchor. Front Microbiol 8:481.
11. Tang W, Wu Y, Li M, Wang J, Mei S, Tang B*, Tang XF* (2016) Alternative translation initiation of a haloarchaeal serine protease transcript containing two in-frame start codons. J Bacteriol 198(13):1892-901. (Spotlight article)
12. Liu F, Zhao ZS, Ren Y, Cheng G, Tang XF, Tang B* (2016) Autocatalytic activation of a thermostable glutamyl endopeptidase capable of hydrolyzing proteins at high temperatures. Appl Microbiol Biotechnol 100(24):10429-41.
13. Meng D, Dai M, Xu BL, Zhao ZS, Liang X, Wang M, Tang XF, Tang B* (2016) Maturation of fibrinolytic bacillopeptidase F involves both hetero- and autocatalytic processes. Appl Environ Microbiol 82(1):318-27.
14. Xu BL, Dai M, Chen Y, Meng D, Wang Y, Fang N, Tang XF, Tang B* (2015) Improving the thermostability and activity of a thermophilic subtilase by incorporating structural elements of its psychrophilic counterpart. Appl Environ Microbiol 81(18):6302-13.
15. Wang L, Cheng G, Ren Y, Dai Z, Zhao ZS, Liu F, Li S, Wei Y, Xiong J, Tang XF, Tang B* (2015) Degradation of intact chicken feathers by Thermoactinomyces sp. CDF and characterization of its keratinolytic protease. Appl Microbiol Biotechnol 99(9): 3949-59.
16. Du X, Li M, Tang W, Zhang Y, Zhang L, Wang J, Li T, Tang B, Tang XF* (2015) Secretion of Tat-dependent halolysin SptA capable of autocatalytic activation and its relation to haloarchaeal growth. Mol Microbiol 96(3):548-65.
17. Zhang Y, Wang M, Du X, Tang W, Zhang L, Li M, Wang J, Tang B, Tang XF* (2014) Chitin accelerates activation of a novel haloarchaeal serine protease that deproteinizes chitin-containing biomass. Appl Environ Microbiol 80(18):5698-708.
18. Zeng J, Gao X, Dai Z, Tang B, Tang XF* (2014) Effects of metal ions on stability and activity of hyperthermophilic pyrolysin and further stabilization of this enzyme by modification of a Ca2+-binding site. Appl Environ Microbiol 80(9):2763-72.
19. Feng J, Wang J, Zhang Y, Du X, Xu Z, Wu Y, Tang W, Li M, Tang B, Tang XF* (2014) Proteomic analysis of the secretome of haloarchaeon Natrinema sp. J7−2. J Proteome Res 13(3):1248-58.
20. Zhu H, Xu BL, Liang X, Yang YR, Tang XF, Tang B* (2013) Molecular basis for auto- and hetero-catalytic maturation of a thermostable subtilase from thermophilic Bacillus sp. WF146. J Biol Chem 288(48):34826-38.
21. Li XG*, Yang J, Wang R, Tang XF, Meng JJ, Qin HJ, Liu X, Guo F, Wan SB* (2013) Effects of ectopically expressed hyperthermophilic archaeon (Pyrococcus furiosus) ribulose-1,5-bisphosphate carboxylase/oxygenase on tobacco photosynthesis. Photosynthetica 51 (3): 387-94.
22. Feng J, Liu B, Zhang Z, Ren Y, Li Y, Gan F, Huang Y, Chen X, Shen P, Wang L, Tang B*, Tang XF* (2012) The complete genome sequence of Natrinema sp. J7-2, a haloarchaeon capable of growth on synthetic media without amino acid supplements. PLoS ONE 7(7):e41621.
23. Dai Z, Fu H, Zhang Y, Zeng J, Tang B, Tang XF* (2012) Insights into the maturation of hyperthermophilic pyrolysin and the roles of its N-terminal propeptide and long C-terminal extension. Appl Environ Microbiol 78(12):4233-41.
24. Xu Z, Du X, Li T, Gan F, Tang B, Tang XF* (2011) Functional Insight into the C-Terminal Extension of Halolysin SptA from Haloarchaeon Natrinema sp. J7. PLoS ONE 6(8):e23562.
25. Liang X, Bian Y, Tang XF, Xiao G, Tang B* (2010) Enhancement of keratinolytic activity of a thermophilic subtilase by improving its autolysis resistance and thermostability under reducing conditions. Appl Microbiol Biotechnol 87:999–1006.
26. Fang N, Zhong CQ, Liang X, Tang XF, Tang B* (2010) Improvement of extracellular production of a thermophilic subtilase expressed in Escherichia coli by random mutagenesis of its N-terminal propeptide. Appl Microbiol Biotechnol 85(5):1473-81.
27. Zhong CQ, Song S, Fang N, Liang X, Zhu H, Tang XF, Tang B* (2009) Improvement of low-temperature caseinolytic activity of a thermophilic subtilase by directed evolution and site-directed mutagenesis. Biotechnol Bioeng 104(5):862-70. (Spotlight article)
28. Cheng G, Zhao P, Tang XF, Tang B* (2009) Identification and characterization of a novel spore-associated subtilase from Thermoactinomyces sp. CDF. Microbiology-SGM 55:3661-72.
29. Zeng C, Zhao YZ, Cui CZ, Zhan H, Zhu JY, Tang XF, Shen P, Huang YP*, Chen XD*. (2009) Characterization of the Haloarcula hispanica amyH gene promoter, an archaeal promoter that confers promoter activity in Escherichia coli. Gene 442: 1–7.
30. Yang YR, Zhu H, Fang N, Liang X, Zhong CQ, Tang XF, Shen P, Tang B* (2008) Cold-adapted maturation of thermophilic WF146 protease by mimicking the propeptide binding interactions of psychrophilic subtilisin S41. FEBS Lett 582(17):2620-6.
31. Shi W, Tang XF, Huang Y, Gan F, Tang B*, Shen P（2006）An extracellular halophilic protease SptA from a halophilic archaeon Natrinema sp. J7: gene cloning, expression and characterization. Extremophiles 10:599–606.
32. Bian Y, Liang X, Fang N, Tang XF, Tang B*, Shen P, Peng Z（2006）The roles of surface loop insertions and disulfide bond in the stabilization of thermophilic WF146 protease. FEBS Lett 580:6007–14. (Faculty 1000 of Biology Recommended)
33. Tang XF, Shen Y, Matsui E, Matsui I* (2004) Domain topology of the DNA polymerase D complex from a hyperthermophilic archaeon Pyrococcus horikoshii. Biochemistry 43(37):11818-27.
34. Shen Y, Tang XF, Yokoyama H, Matsui E, Matsui I* (2004) A 21-amino acid peptide from the cysteine cluster II of the family D DNA polymerase from Pyrococcus horikoshii stimulates its nuclease activity which is Mre11-like and prefers manganese ion as the cofactor. Nucleic Acids Res 32:158-68.
35. Shen Y, Tang XF, Matsui E, Matsui I* (2004) Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcus horikoshii. Biochem Soc Trans 32:245-9.
36. Shen Y, Tang XF, Matsui I* (2003) Subunit interaction and regulation of activity through terminal domains of family D DNA polymerase from Pyrococcus horikoshii. J Biol Chem 278: 21247-57.
37. Tang XF, Ezaki S, Atomi H, Imanaka T* (2001) Anthranilate synthase without an LLES motif from a hyperthermophilic archaeon is inhibited by tryptophan. Biochem Biophys Res Commun 281:858-65.
38. Matsuda T, Fujikawa M, Haruki M, Tang XF, Ezaki S, Imanaka T, Morikawa M, Kanaya S* (2001) Interaction of TIP26 from a hyperthermophilic archaeon with TFB/TBP/DNA ternary complex. Extremophiles 5:177-82.
39. Tang XF, Ezaki S, Atomi H, Imanaka T* (2000) Biochemical analysis of a thermostable tryptophan synthase from a hyperthermophilic archaeon. Eur J Biochem 267:6369-77.
40. Tang XF, Ezaki S, Fujiwara S, Takagi M, Atomi H, Imanaka T* (1999) The tryptophan biosynthesis gene cluster trpCDEGFBA from Pyrococcus kodakaraensis KOD1 is regulated at the transcriptional level and expressed as a single mRNA. Mol Gen Genet 262:815-21.
